Presence of two polypeptide chains comprising fatty acid synthetase.
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چکیده
منابع مشابه
Presence of two polypeptide chains comprising fatty acid synthetase.
Highly purified fatty acid synthetases of chicken and rat livers have molecular weights of 500,000 and dissociate in solutions of low ionic strength into subunits of molecular weight 250,000 with loss of synthetase activity. The subunits can be reassociated in phosphate buffer with full restoration of the activity. In the presence of sodium dodecyl sulfate or guanifine-HCl, the synthetases diss...
متن کاملRegulation of synthesis of hepatic fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes.
Mammalian fatty acid synthetase was shown to be composed of two peptides, molecular weight 240,000, after dissociation with sodium dodecyl sulfate. Rat liver polysomes that synthesize fatty acid synthetase were identified by sucrose gradient analysis of polysomes that had been reacted with 125I-labeled antibody against fatty acid synthetase. The binding of 125I-labeled antibody to polysomes was...
متن کاملGoose fatty acid synthetase mRNA.
The fatty acid synthetase of animal tissues consists of two identical subunits (Mr = 250,000), each of which is a multienzyme protein containing domains for the acyl carrier peptide and the seven different catalytic activities required for the conversion of acetyl-CoA and malonyl-CoA to palmitate. Total poly(A+) RNA was isolated from goose uropygial gland and translated in a cell-free rabbit re...
متن کاملRecombination of Antibody Polypeptide Chains in the Presence of Antigen
Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially inter...
متن کاملPresence of Two Types of L Polypeptide Chains in Guinea Pig 7s Immunoglobulins
Guinea pig 7S immunoglobulins (gamma(1) and gamma(2)) consist of two groups of molecules (K and L) bearing different types of L chains (kappa and lambda). Approximately one-third of the molecules in normal guinea pig gamma(2)-globulin bear the lambda- type of L chains, and all or most of the others bear kappa-chains. kappa- and lambda-chains have similar molecular weights.
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1975
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.72.5.1940